IBSW 98 TUTORIAL: PROTEIN AND PEPTIDE STRUCTURE*

6/16/98


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Table of Contents

PROTEIN AND PEPTIDE STRUCTURE*

AA Stereochemistry, see also tutorial material

Peptide Definition

Other Definitions

More on Amino Acids, see also tutorial material

2 Unusual ones

Others by modification

Formation of peptide

Peptide nomenclature

Proteins fold into compact structures.

rotation about main-chain bonds...

THREE repeating torsion angles

OMEGA angle tends to be planar

Trans is generally favored over cis:

Leaves phi and psi for flexible folding...

When phi/psi torsion angles repeat

Repeating Phi= -57, Psi= -47 gives...

Stability of helical structure

Cytochrome b562 is mostly helix

Phi = -119, Psi = +113 gives...

strands “twist”...

Phi= -139, Psi=+135 gives...

Plastocyanin is mostly sheet

These structural elements defined by...

another kind of secondary structure

Proline is often found in turns...

...sometimes with cis peptide bonds

Classification of Protein Folding:

Quaternary structure

"Tertiary" Structure

Tertiary structure...

by covalent crosslinks,

by non-bonding interactions,

by non-bonding interactions,

"Quaternary" Structure

e.g. the two chains of insulin

More subunits give more symmetry choices...

Thus...

Author: John E. Wampler

Email: wampler@bchiris.bmb.uga.edu

Home Page: http://bmbiris.bmb.uga.edu/wampler

This presentation is also available on-line at http://bmbiris.bmb.uga.edu/wampler/isbw98/index.html

* Additional tutorial material at http://bmbiris.bmb.uga.edu/wampler/tutorial

REFERENCES:

R. L. Almassy, C. A. Janson and D. Eisenberg, Nature 323, 304-309 (1986)

T. E. Creighton, Proteins, Structures and Molecular Properties, W. H. Freeman and Co., N.Y. (1993).

A. L. Lehninger, Biochemistry, Worth Publishers, Inc., N.Y. (1975)

A. L. Lehninger, D. L. Nelson, M.M. Cox, Principles of Biochemistry, Worth Publishers, Inc., N. Y. (1993).

D. L. Oxender and C. F. Fox, Protein Engineering, Alan R. Liss, Inc., N. Y. (1987)

J. S. Richardson & D. C. Richardson, in Prediction of Protein Structure and the Principles of Protein Conformation (G. D. Fasman, ed.), Plenum Press, N.Y. (1989) pp. 1-98.

D. E. Stewart, A. Sarkar and J. E. Wampler, J. Mol. Biol 214, 253-260 (1990)

E. Tronrud, M. Schmid and B. Matthews, J. Med. Biol. 188, 443 (1986)

K. S. Wilson, C. E. Vorgias, I. Tanaka, S. W. White and M. Kimura, Protein Engineering 4, 11-22 (1990).