METALLOPROTEIN MODELING & COMPUTATIONAL CHEMISTRY

7/31/2000


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NOTICE: This material is copyrighted and all right are reserved. (c) 1998, 2000 J. E. Wampler
Students may make a single copy for study purposes.

Table of Contents

1) METALLOPROTEIN MODELING & COMPUTATIONAL CHEMISTRY

2) Some exciting & rapidly developing areas...

3) Isopotential maps...

4) Brownian Dynamics calculations...

5) Protein docking...

6) Comparison of Docking and Brownian Dynamics

7) MOLECULAR MECHANICS is the workhorse of Computational Biochemsitry

8) The Molecular Mechanics description of a structure consists of...

9) Molecular Mechanics Force Field Or Potential Functions

10) What about the ellipsis, …?

11) Force fields may be classified by...

12) A Survey & Comparison of some Force Field Functions

13) Some Bond Stretch Functions:

14) Model bond as spring...

15) Harmonic is simplest function, but...

16) However, only fit to bottom of “well” is important.

17) Similarly, Bond Angle Functions

18) Torsion Angle Functions

19) Torsion Angle Functions...

20) Some other torsion potentials...

21) NON-BONDING INTERACTIONS

22) Other combination rules...

23) Hill potentials

24) Buffered-14-7 potential (Halgren, 1992)

25) Soft versus Hard potentials

26) Electrostatic interactions

27) Monopole models...

28) Multipole models...

29) MM2 dipole-dipole potential:

30) Fixes for Coulombic Potentials

31) Solvent screening...

32) Each force field has added functions...

33) To get this potential

34) By evaluating it,

35) Example...

36) Such maps help explain the Ramachandran plots

37) MM offers a method of minimizing the energy toward the optimum geometry

38) Example… the Lysyl-aspartate dipeptide

39) Molecular Dynamics

40) The MD Process…

41) Process...EQUILIBRATION

42) The result is ...

43) Frame-by-frame examination...

44) A variety of trajectories from this data

45) Issues for Metals and Metalloproteins

46) Appropriate Experimental data is limited

47) Three general MM approaches…(see Hay, 1993)

48) Three general MM approaches…Pros and Cons:

49) Are charges needed?

50) What’s been done?

51) Read more about it…

Author: John E. Wampler

Email: wampler@bmb.uga.edu

Home Page: http://bmbiris.bmb.uga.edu/wampler/ibsw00

For References See:

http://bmbiris.bmb.uga.edu/wampler/ibsw00/ref.html

AND

http://bmbiris.bmb.uga.edu/wampler/ibsw00/morerefs.html