Professor and Ramsey/Georgia Research Alliance (GRA) Eminent Scholar in Structural Biology B.Sc. in Chem. Engineering (1960), Cheng Kung University, Taiwan Ph. D. in Chemistry (1968), University of Arkansas Member of the Center for Metalloenzyme Studies Office: B204A Life Sciences, Contact Phone Number: 542-1747 Lab: B202, B212A, A210 Life Sciences, Lab Phone: 542-3384 / 542-1086 E-mail: wang@bcl1.bmb.uga.edu Additional Information / Complete Resume See also: Ser-Cat, Georgia X-Ray Crystallography Laboratory (GXRCC)

Research Interests

Application of structural biology tools for the structure-function studies of relevant biological macromolecules, which include RNA polymerases, neurophysins and their hormone complexes, glutathione transferases, glutamine binding protein and complex, aldehyde dehydrogenase, augmenter of liver regeneration, green fluorescent protein, hemerythrin, myohemerythrin, ferrochelatase and plant cell wall polysaccharides. Application of multidisciplinary methodologies for structural genomics and structural proteomics.

Development of advanced instrumentation for diffraction studies including synchrotron X-ray sources, remote access for beamline operations, robotic sample mounting technologies, and in-house X-ray sources. Development of computer software packages for Direct Crystallography - phasing from unlabelled native crystals, signal-based data collection, cost-effective high-throughput structure determination pipelines, and refinement methods to ensure high-quality structural results.

Selected Recent Publications

• Shaw, N.; Tempel, W.; Chang, J.; Yang, H.; Cheng, C.; Ng, J.; Rose, J.; Rao, Z.; Wang, B. C.; Liu, Z. J., Crystal structure solution of a ParB-like nuclease at atomic resolution. Proteins 2008, 70, (1), 263-7.



• Bahti, P.; Chen, S.; Li, Y.; Shaw, N.; Zhang, X.; Zhang, M.; Cheng, C.; Song, G.; Yin, J.; Zhang, H.; Che, D.; Abbas, A.; Xu, H.; Wang, B. C.; Liu, Z. J., Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304. Acta Crystallogr Sect F Struct Biol Cryst Commun 2008, 64, (Pt 2), 91-3.



• Xie, Y.; Takemoto, C.; Kishishita, S.; Uchikubo-Kamo, T.; Murayama, K.; Chen, L.; Liu, Z. J.; Wang, B. C.; Manzoku, M.; Ebihara, A.; Kuramitsu, S.; Shirouzu, M.; Yokoyama, S., Structure of the minimized alpha/beta-hydrolase fold protein from Thermus thermophilus HB8. Acta Crystallogr Sect F Struct Biol Cryst Commun 2007, 63, (Pt 12), 993-7.



• Shimada, A.; Niwa, H.; Tsujita, K.; Suetsugu, S.; Nitta, K.; Hanawa-Suetsugu, K.; Akasaka, R.; Nishino, Y.; Toyama, M.; Chen, L.; Liu, Z. J.; Wang, B. C.; Yamamoto, M.; Terada, T.; Miyazawa, A.; Tanaka, A.; Sugano, S.; Shirouzu, M.; Nagayama, K.; Takenawa, T.; Yokoyama, S., Curved EFC/F-BAR-domain dimers are joined end to end into a filament for membrane invagination in endocytosis. Cell 2007, 129, (4), 761-72.



• Shaw, N.; Zhao, M.; Cheng, C.; Xu, H.; Saarikettu, J.; Li, Y.; Da, Y.; Yao, Z.; Silvennoinen, O.; Yang, J.; Liu, Z. J.; Wang, B. C.; Rao, Z., The multifunctional human p100 protein 'hooks' methylated ligands. Nat Struct Mol Biol 2007, 14, (8), 779-84.



• Shaw, N.; Tempel, W.; Chang, J.; Yang, H.; Cheng, C.; Ng, J.; Rose, J.; Rao, Z.; Wang, B. C.; Liu, Z. J., Crystal structure solution of a ParB-like nuclease at atomic resolution. Proteins 2007, 70, 1, 263-267.



• Shaw, N.; Cheng, C.; Tempel, W.; Chang, J.; Ng, J.; Wang, X. Y.; Perrett, S.; Rose, J.; Rao, Z.; Wang, B. C.; Liu, Z. J., (NZ)CH...O contacts assist crystallization of a ParB-like nuclease. BMC Struct Biol 2007, 7, 46.



• Liu, Z. J.; Chen, H.; Shaw, N.; Hopper, S. L.; Chen, L.; Chen, S.; Cerniglia, C. E.; Wang, B. C., Crystal structure of an aerobic FMN-dependent azoreductase (AzoA) from Enterococcus faecalis. Arch Biochem Biophys 2007, 463, (1), 68-77.



• Kondo, N.; Nakagawa, N.; Ebihara, A.; Chen, L.; Liu, Z. J.; Wang, B. C.; Yokoyama, S.; Kuramitsu, S.; Masui, R., Structure of dNTP-inducible dNTP triphosphohydrolase: insight into broad specificity for dNTPs and triphosphohydrolase-type hydrolysis. Acta Crystallogr D Biol Crystallogr 2007, 63, (Pt 2), 230-9.



• Kelley, L. L.; Dillard, B. D.; Tempel, W.; Chen, L.; Shaw, N.; Lee, D.; Newton, M. G.; Sugar, F. J.; Jenney, F. E., Jr.; Lee, H. S.; Shah, C.; Poole, F. L., 3rd; Adams, M. W.; Richardson, J. S.; Richardson, D. C.; Liu, Z. J.; Wang, B. C.; Rose, J., Structure of the hypothetical protein PF0899 from Pyrococcus furiosus at 1.85 A resolution. Acta Crystallogr Sect F Struct Biol Cryst Commun 2007, 63, (Pt 7), 549-52.



• Kanaujia, S. P.; Ranjani, C. V.; Jeyakanthan, J.; Baba, S.; Chen, L.; Liu, Z. J.; Wang, B. C.; Nishida, M.; Ebihara, A.; Shinkai, A.; Kuramitsu, S.; Shiro, Y.; Sekar, K.; Yokoyama, S., Crystallization and preliminary crystallographic analysis of molybdenum-cofactor biosynthesis protein C from Thermus thermophilus. Acta Crystallogr Sect F Struct Biol Cryst Commun 2007, 63, (Pt 1), 27-9.



• Gerwe, B.; Clancy Kelley, L. L.; Dillard, B. D.; Lai, T.; Liu, Z. J.; Tempel, W.; Chen, L.; Habel, J.; Lee, D.; Jenney, F. E. J.; Sugar, F. J.; Richardson, J. S.; Richardson, D. C.; Newton, M. G.; Wang, B. C.; Adams, M. W. W.; Rose, J. P., Structural and Transcriptional Analyses of a Purine Nucleotide-Binding Protein from Pyrococcus furiosus:  a Component of a Novel, Membrane-Bound Multiprotein Complex Unique to this Hyperthermophilic Archaeon. J Struct Funct Genomics 2007, 8, 1, 1-10.



• Fu, Z. Q.; Chrzas, J.; Sheldrick, G. M.; Rose, J. P.; Wang, B. C., A Parallel Program Using SHELXD for Quick Heavy-Atom Partial Structural Solution on High-Performance Computers. J. Appl  Cryst 2007, 40, 387-390.



• Das, A.; Fu, Z. Q.; Tempel, W.; Liu, Z. J.; Chang, J.; Chen, L.; Lee, D.; Zhou, W.; Xu, H.; Shaw, N.; Rose, J. P.; Ljungdahl, L. G.; Wang, B. C., Characterization of a corrinoid protein involved in the C1 metabolism of strict anaerobic bacterium Moorella thermoacetica. Proteins 2007, 67, (1), 167-76.



• Cacciapuoti, G.; Porcelli, M.; Moretti, M. A.; Sorrentino, F.; Concilio, L.; Zappia, V.; Liu, Z. J.; Tempel, W.; Schubot, F.; Rose, J. P.; Wang, B. C.; Brereton, P. S.; Jenney, F. E.; Adams, M. W., The first agmatine/cadaverine aminopropyl transferase: biochemical and structural characterization of an enzyme involved in polyamine biosynthesis in the hyperthermophilic archaeon Pyrococcus furiosus. J Bacteriol 2007, 189, (16), 6057-67.



• Wang, Y.; Li, W.; Zhang, T.; Ding, C.; Lu, Z.; Long, N.; Rose, J. P.; Wang, B. C.; Lin, D., Reconstruction of ancient genome and gene order from complete microbial genome sequences. J Theor Biol 2006, 239, (4), 494-8.



• Ruble, J. R.; Wang, B. C.; Rose, J. P., A simple method for motorized alignment of Osmic confocal optics. J. Appl. Cryst. 2006, 39, 892-894.



• Rose, J. P.; Liu, Z. J.; Chen, L.; Lee, D.; Tempel, W.; Newton, M. G.; Wang, B. C., High throughput de novo structure determination on a home source using quick soaks, ACTOR and parameter space screening. Rigaku J. 2006, 23, 3-12.



• Liu, Z. J.; Stepanyuk, G. A.; Vysotski, E. S.; Lee, J.; Markova, S. V.; Malikova, N. P.; Wang, B. C., Crystal structure of obelin after Ca2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited state. Proc Natl Acad Sci U S A 2006, 103, (8), 2570-5.



• Kuratani, M.; Sakai, H.; Takahashi, M.; Yanagisawa, T.; Kobayashi, T.; Murayama, K.; Chen, L.; Liu, Z. J.; Wang, B. C.; Kuroishi, C.; Kuramitsu, S.; Terada, T.; Bessho, Y.; Shirouzu, M.; Sekine, S.; Yokoyama, S., Crystal structures of tyrosyl-tRNA synthetases from Archaea. J Mol Biol 2006, 355, (3), 395-408.